Bacteroides is a genus of gramnegative, obligate anaerobic, nonsporulating bacilli that belong to the family bacteroidaceae. The slayerlike antigens were recently isolated from b. Pdf characterization of bacteroides forsythus researchgate. Immunoglobulin g and a antibody responses to bacteroides. Fifteen bacteroides forsythus strains freshly isolated from patients with periodontitis were used together with three collection strains and one type strain for characterization of growth on various media. Organisms are part of the normal flora of the oral, respiratory, intestinal, and. Quantitation of bacteroides forsythus in subgingival. Bacteroides vulgatus and bacteroides dorei reduce gut. Bacteroides nodosus definition of bacteroides nodosus by. The genus bacteroides includes some of the predominant gut bacteria in humans and is known to have an important role in maintaining a healthy gut ecosystem. To date, a few bacteria, including bacteroides forsythus, have been.
Article information, pdf download for identification of bacteroides. Selected characteristics of pathogenic and nonpathogenic strains of bacteroides gingivalis. The characteristics of the fusiform species bacteroides forsythus, isolated from human periodontal pockets, were examined. Bacteroides forsythus, which has been reported to be associated with periodontitis but has not been recognized as a key pathogen, was found to induce cytolytic activity against hl60 and other human leukemic cells. Tannerella forsythia is an anaerobic, gramnegative bacterial species of the cytophagabacteroidetes family. Bacteroides have been included in bergeys manual of. Pdf porphyromonas gingivalis, bacteroides forsythus and other. Nov 01, 2002 read porphyromonas gingivalis, bacteroides forsythus and other putative periodontal pathogens in subjects with and without periodontal destruction, journal of clinical periodontology on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Quantitation of bacteroides forsythus in subgingival plaque.
Bacteroides forsythus is a fastidious gramnegative, anaerobic, nonmotile, nonsporeforming, spindleshaped, highly pleomorphic rod, first reported as a fusiform bacteroides and described as species in 1986 by tanner et al. Tannerella forsythia formerly bacteroides forsythus, together with porphyromonas gingivalis and treponema denticola, are considered to be the major. Antigenic variation and crossreactivity in bacteroides. Shared antigens of porphymmonas gingivalis and bacteroides forsythus. Bacteroides forsythus definition of bacteroides forsythus. Prevalence of bacteroides forsythus and bacteroides gingivalis in subgingival plaque of prosthodontically treated patients on short recall. Pdf shared antigens of porphymmonas gingivalis and. The aim of this study was to identify the major strainvariable and crossreactive antigens of b. Bacteroides forsythus is a gramnegative, anaerobic, fusiform bacterium and is. Detection of actinobacillus actinotnycetemcomitans, porphyromonas gingivalis, and bacteroides forsythus in an a. Interdental supragingival plaquea natural habitat of actinobacillus actinomycetemcomitans, bacteroides forsythus, campylobacter rectus, and prevotella nigrescens r. Bacteroides is a genus of gramnegative, obligate anaerobic bacteria. Bacteroides forsythus is one of the important periodontopathic bacteria, and this microorganism is known to have an slayer outside the outer membrane.
The etiology is clearly bacterial, and a small number of bacterial species have been consistently associated with periodontitis, including bacteroides forsythus and porphyromonas gingivalis. Bacteroides forsythus is one of the etiologic agents of destructive periodontal diseases. The orf tf0022 of strain atcc 43037 encodes a hybrid twocomponent system consisting of an nterminal histidine kinase and a cterminal response regulator. Detection of bacteroides forsythus and porphyromonas. Simultaneous detection of bacteroides forsythus and prevotella intermedia by 16s rrna genedirected multiplex pcr. Pdf fifteen bacteroides forsythus strains freshly isolated from patients with periodontitis were used together with. Bacteroides strains demonstrating higher or resistant mics were correlated with treatment failure and a trend towards increased mortality 78,102. Organisms are part of the normal flora of the oral, respiratory, intestinal, and urogenital cavities of humans and animals. It has been implicated in periodontal diseases and is a member of the red complex of periodontal pathogens. Interdental supragingival plaquea natural habitat of.
Chronic periodontitis is a common infectious disease in the adult population. Analysis of the cell surface layer ultrastructure of the oral pathogen. Characterization of bacteroides forsythus isolates. The aim of this study was to isolate the slayer from b. Identification of bacteroides forsythus in subgingival dental plaque. Bacteroides is the predominant genus within the lower human intestinal tract, as evidenced by its prevalence in the product of this openended culture system, faeces. Bacteroides forsythus has been shown to be prevalent among patients. Characterization of the trypsinlike activity of bacteroides forsythus daniel grenier tel. In this study, these proteins were confirmed to be specific to b. Anaerobic culture to detect periodontal and caries pathogens. Many oral bacterial species have been suspected to be associated with periodontal disease. Detection of actinobacillus actinotnycetemcomitans. Comparison of immunoassay and quantitative polymerase chain reaction. The phylogenetic structure of the bacteroides subgroup of the cytophagaflavobacter bacteroides cfb phylum was examined by 16s rrna sequence comparative analysis.
The analysis requires less than 4 hrs to perform, and is specific only to b. Comparatively little attention has been paid to the identification of healthassociated and potentially beneficial bacterial. This cytolytic activity was demonstrated according to three different criteria. Unusual in bacterial organisms, bacteroides membranes contain sphingolipids. Fluorescence in situ hybridization for direct visualization of gram. Molecular detection of bacteroides forsythus in human. Pcr was used to delineate the genetic diversity of b.
The slayer has been shown to be composed of at least two high molecular weight glycoproteins of 220 and 210 kda size encoded by the. Porphyromonas gingivalis, bacteroides forsythus and other. Bacteroides forsythus is a fastidious anaerobic gramnegativeorganism associated with various forms of periodontal disease. Bacteroides forsythus synonyms, bacteroides forsythus pronunciation, bacteroides forsythus translation, english dictionary definition of bacteroides forsythus. The presence of dna was confirmed for all negative samples by use of universal prokaryotic primers. Jan 01, 2003 read endpoint quantitative pcr assays for bacteroides forsythus, porphyromonas gingivalis, and actinobacillus actinomycetemcomitans, journal of periodontal research on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Reclassification of bacteroides forsythus tanner et al.
A common feature of these organisms is the production of high levels of proteolytic activity. Bacteroides forsythus has emerged as a crucial periodontal pathogen with possible implications for systemic disease. The surface s layer is a virulence factor of bacteroides. Article information, pdf download for interdental supragingival. Endpoint quantitative pcr assays for bacteroides forsythus. Tannerella forsythia formerly bacteroides forsythus, together with porphyromonas gingivalis and treponema denticola, are considered to be the major periodontal pathogens 1. Pdf porphyromonas gingivalis, bacteroides forsythus and. Association of bacteroides forsythus and a novel bacteroides phylotype with periodontitis. Novel apoptosisinducing activity in bacteroides forsythus.
Approximately 95% of the 16s rrna sequence was determined for 36 representative strains of species of prevotella, bacteroides, and porphyromonas and related species by a modified sanger sequencing method. Bacteroides fragilis bacteroides merdae bacteroides ovatus bacteroides thetaiotaomicron bacteroides uniformis bacteroides vulgatus nonpigmented group bacteroides capillosus bacteroides coagulans bacteroides forsythus. Bacteroides forsythus in primary endodontic infections as. The study was accomplished with a set of samples that were collected from subjects with periodontitis and healthy controls. Determining which antigenic components of the bacterium are recognized in the immune response of periodontitis patients is an important step in assessing strategies for vaccine development. Bacteroides infectious disease and antimicrobial agents. Association of bacteroides forsythus and a novel bacteroides. Pdf simultaneous detection of bacteroides forsythus and. Bacteroides species and a new species bacteroides forsythus was proposed 10. Tannerella forsythia is a gramnegative oral anaerobe closely associated with both periodontal and periapical diseases.
Detection of tannerella forsythensis bacteroides forsythus. Bacteroides species are non endospore forming bacilli, and may be either motile or nonmotile, depending on the species. Characterization of bacteroides forsythus article pdf available in journal of clinical microbiology 356. Identification of bacteroides forsythus in subgingival dental. Bacteroides definition of bacteroides by medical dictionary. In addition, the arbitrarily primed polymerase chain reaction ap. The bspa gene encoding a cell surface associated leucinerich repeat protein bspa involved in adhesion to fibronectin and fibrinogen was recently cloned from this bacterium in our laboratory. It was initially named bacteroides forsythus tanner et al. The purpose of the present study was to examine the relationship of the presence of b. Bacteroides nodosus synonyms, bacteroides nodosus pronunciation, bacteroides nodosus translation, english dictionary definition of bacteroides nodosus.810 1363 459 1298 208 1588 136 463 907 924 981 1067 498 1328 1202 1590 1548 1432 934 101 498 887 941 1586 293 305 762 1449 989 1289 385 753 760 1270 674 1574 298 451 1587 250 960 986 1456 292 899 1041 1219